Identification of N-glycan oligomannoside isomers in the diatom Phaeodactylum tricornutum

نویسندگان

چکیده

• P. tricornutum oligomannoside isomers are identical to the mammalian ones. synthesize a canonical Man 5 GlcNAc 2, substrate for GnT I. exhibits N -glycan profile that is suitable production of human biopharmaceuticals. Microalgae emerging systems recombinant proteins like monoclonal antibodies. In this context, characterization host cell N- glycosylation machinery and microalgae-made biopharmaceuticals, which mainly glycoprotein-based products, requires efficient analytical methodologies dedicated profiling -glycans. Herein, in order gain knowledge regarding its -glycosylation pathway, we protein -linked oligosaccharides isolated from diatom Phaeodactylum has been used successfully produce functional The combination ion mobility spectrometry–mass Spectrometry electrospray ionization-multistage tandem mass spectrometry allows us decipher detailed structure demonstrate processing oligomannosides occurs as reported mammals. Therefore, synthesizes human-like contrast other microalgae species. This represent an advantage alternative ecofriendly expression system biopharmaceuticals therapy.

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ژورنال

عنوان ژورنال: Carbohydrate Polymers

سال: 2021

ISSN: ['1879-1344', '0144-8617']

DOI: https://doi.org/10.1016/j.carbpol.2021.117660